TABLE 5.6

Overview on static cultivation vessels for adherent (and suspension) cells

Vessel

Supplier

Area (cm2)

Average cell

yield

Working

volume (L)

Cells/volume

(normalized to

T175)

Growth support

matrix

Control options

Perfusion

options

T175

e.g., Corning

1.8E02

1.8E07

0.05

1.0

Bottom inner wall

Offline

No

RB

e.g., Corning

8.5E02

1.8E03

8.5E07

1.8E08

0.26

0.53

0.9

0.9

Inner wall

Offline

No

CellStack

Corning

6.4E02

2.5E04

6.4E07

2.5E09

0.2

7.6

0.9

0.9

Trays

Offline

No

HYPERFlask

Corning

1.7E03

1.9E08

0.6

0.8

Trays

Offline

No

HYPERStack

Corning

6.0E03

6.0E04

4.0E09

4.0E10

1.2

12.0

9.3

9.3

Trays

Offline

No

Cell Factory

Nunc

6.3E02

2.5E04

0.2

8.0

Trays

Offline

No

CellCube

Corning

8.5E03

8.5E04

8.5E08

8.5E09

0.6

6.0

3.9

3.9

Plates

Offline

Yes

Tide Motion

CelCradle

Esco VacciXell

1.6E04

5.5E09

0.5

30.6

Fluidized bed

macrocarriers

Offline

Alternate

submerging

TideXcell

Esco VacciXell

3.2E05

1.5E06

1.6E07

7.0E10

3.5E11

3.5E12

2.0

20.0

100.0

97.2

97.2

97.2

Fluidized bed with

matrix cassettes

macrocarriers

Off- and online

control

Yes

CelliGen

BioBLU 5p

Eppendorf

3.0E04

6.0E05

2.5E10

5.0E11

5.0

50.0

13.9

27.8

Fixed

bedmacrocarriers

Off- and online

control

Yes

iCellis

Pall

5.3E03

5.0E06

1.5E09

1.5E12

1.0

70.0

4.2

59.5

Fixed

bedmacrocarriers

Off- and online

control

Yes

Scale-X

Hydro

Carbo

Nitro

Univercells

technologies

2.4E04

3.0E05

6.0E06

0.8

3.3

60.0

Fixed bed sheets of

non-woven PET

fabric

Off- and online

control

Yes

Adapted from [ 2]. Additional information was included from the websites from Univercells technologies and Esco VacciXell.

104

Bioprocessing of Viral Vaccines